FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6639-t6hop1-160326.35100000057840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6639-t6hop1-160326.35100000057840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22311563
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1345460.6030326069043213TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC161900.041.01121513
GTCACGC158250.040.9614229
TCACGCC157850.040.88037530
AGTCACG160000.040.65402228
ACGTCTG163850.040.55050715
CACGTCT165100.040.1618214
TATCTCG166550.039.04239339
GAACTCC168700.038.87737721
GATCGGA173400.038.7823751
CGTCTGA172800.038.42419416
AGCACAC173550.038.38779410
GAGCACA175600.038.0675139
TCCAGTC175100.037.53348525
CAGTCAC175200.037.39665227
CACACGT179900.037.3582312
GTCTGAA178100.037.35629717
ATCTCGT174350.037.15377440
CCAGTCA177450.036.90979426
CTCGTAT176800.036.58891342
AGAGCAC184000.036.317428
Produced by FastQC (version 0.11.2)