FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6639-t3sir3-160326.3510000005790b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6639-t3sir3-160326.3510000005790b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21620197
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG837520.38737852388671573TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC102600.039.342113
ACGTCTG103150.039.06670815
CACGTCT105550.038.199914
CGTCTGA107650.037.39183416
GATCGGA111900.036.5259741
GAACTCC109650.036.27805721
AGCACAC113300.035.9048410
GTCACAG110350.035.68100729
TCCAGTC110200.035.64782325
CACAGTC110300.035.47304531
GAGCACA116550.034.8257649
AGTCACA113900.034.70718828
CACACGT118450.034.41982312
CAGTCAC114450.034.3831327
GTCTGAA117850.034.36491817
CCAGTCA114600.034.2399626
ATCTCGT115850.033.93082442
AGAGCAC121050.033.586898
CTCGTAT118250.033.43244644
TCGGAAG125950.032.5657353
Produced by FastQC (version 0.11.2)