FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6639-t3input-160326.35100000057afb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6639-t3input-160326.35100000057afb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14652076
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG241710.164966384285749TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC33500.034.1193533
ACGTCCG34900.032.4927932
GCACACG37650.031.25461211
AGTCACG37000.030.5870628
CACGTCC38200.029.45011731
ACACGTC40250.029.34738213
GTCCGCA38650.029.2237634
GATCGGA41650.028.6947331
CACACGT41900.028.2457812
CCGCACA40400.028.06964736
ACGTCTG42300.027.97829815
TCACGTC41600.027.2052130
CACGTCT43950.026.97911314
CATCTCG43000.026.84334841
GTCACGT42150.026.74309229
TCCGCAC42900.026.3289935
CGTCTGA44750.026.2956916
AGCACAC46500.025.451410
CAGTCAC47400.024.4455827
GAGCACA49700.023.8126819
Produced by FastQC (version 0.11.2)