FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6639-t3hop1-160326.351000000577fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6639-t3hop1-160326.351000000577fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19246366
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC4581062.3802207647926887TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG508850.043.8721611
ACACGTC514400.043.38984713
GATCGGA521650.043.384071
CACACGT515950.043.23824712
CACGTCT517100.043.08931714
ACGTCTG517450.043.02515815
AGCACAC520150.042.93636710
GAGCACA525500.042.610349
GTCACAC513150.042.521629
CGTCTGA524900.042.3973516
GATCTCG514600.042.3422239
TCGGAAG534950.042.2988053
AGAGCAC531200.042.203948
CACACAG517400.042.20330431
CAGTCAC520650.042.1294627
GAACTCC526100.042.0691621
TCCAGTC523350.042.04097725
GTATGCC520900.042.0075445
CGTATGC525250.041.6684344
CCAGTCA527200.041.66578726
Produced by FastQC (version 0.11.2)