FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6179-t6sir3-160326.351000000578c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6179-t6sir3-160326.351000000578c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20226441
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2478291.2252724045718177TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC282650.043.0252213
CACGTCT286650.042.4405314
ACGTCTG289800.041.95582615
GATCGGA297250.041.552531
AGCACAC294350.041.46056710
CGTCTGA293900.041.339916
GAGCACA295900.041.3344279
CACACGT296500.041.11419312
GAACTCC295400.040.92403421
CAGTCAC297600.040.41757627
TCCAGTC299500.040.30370325
AGAGCAC305300.040.164948
GTATGCC296400.040.0597145
GTCTGAA303850.039.91192217
CCAGTCA301650.039.9047626
TCGGAAG309100.039.90383
CTCGTAT299000.039.62858242
ATCTCGT299700.039.5285240
TCTCGTA307300.038.64600841
TGAACTC315750.038.4076320
Produced by FastQC (version 0.11.2)