FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6179-t6input-160326.35100000057a74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6179-t6input-160326.35100000057a74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18369597
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1197600.6519468010103868TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG147550.042.13338535
GTCCCGA145750.041.2180436
GCACACG162050.040.7646211
CCCGTCC158050.040.2877533
ACCCGTC159400.040.13004732
ACACGTC165050.040.0238913
TCACCCG160600.040.01210830
GATCGGA167650.039.7238581
CACACGT166600.039.69214212
CACCCGT161650.039.6269431
CCCGATC149950.039.52293838
CCGTCCC161400.039.3400234
CACGTCT167100.039.31743214
ACGTCTG167650.039.2420215
TCCCGAT154150.038.5044837
CGTCTGA171200.038.3100216
GTCACCC169000.038.19589229
CGATCTC155100.037.9646140
AGCACAC175550.037.73261610
CCGATCT156000.037.68789339
Produced by FastQC (version 0.11.2)