FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6179-t6hop1-160326.35100000057791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6179-t6hop1-160326.35100000057791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18970150
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC873560.4604918780294304TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG106550.040.2692411
ACACGTC110900.038.7910413
CACACGT112750.038.0551812
ACGTCTG112450.038.0361515
CACGTCT113950.037.6342814
GATCGGA117400.036.7129141
AGCACAC117850.036.5227810
CGTCTGA118050.036.32710616
ACTGACC118100.035.3596332
GAACTCC120650.035.22704321
CAGTCAC119250.035.18779427
GAGCACA122650.035.0749979
TCCAGTC122000.034.560725
CGTATGC121700.034.4076444
GTATGCC122200.034.28508445
CCAGTCA124000.033.91245726
TCGGAAG129250.033.370713
ACTCCAG127500.033.21101823
AGAGCAC130200.033.0929228
CTCGTAT126450.033.06167242
Produced by FastQC (version 0.11.2)