FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6179-t3sir3-160326.35100000057890.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6179-t3sir3-160326.35100000057890.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17048803
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27100.023.49617611
GATCGGA29550.021.8599741
ACACGTC29600.021.8158813
CACACGT31150.020.36930312
CACGTCT32100.020.11682314
ACGTCTG32900.019.55921615
CGTCTGA35050.018.35943416
CATCTCG33550.018.04071639
AGCACAC36400.017.67846910
CGTATGC38400.015.99684544
CAGTCAC40150.015.46659127
TCCAGTC42050.015.30271725
GAACTCC41400.015.10820921
CCAGTCA42650.015.08752726
GAGCACA42850.015.0171529
GTATGCC41300.014.92797545
ATCTCGT43800.014.48668440
AGAGCAC47950.013.9829898
AGTCACC45200.013.78835528
CTCGTAT43850.013.7520742
Produced by FastQC (version 0.11.2)