FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6179-t3input-160326.351000000579fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6179-t3input-160326.351000000579fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18505737
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG909080.4912422563878434TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG119300.038.70046211
GATCGGA125550.037.3952561
ACACGTC124200.037.11928613
CACGTCT125600.036.74136414
ACGTCTG126250.036.71219615
CACACGT126200.036.6740512
CGTCTGA127350.036.2537516
AGCACAC130000.035.61915610
CAGTCAC131100.034.5469527
GAGCACA134950.034.545969
GAACTCC133450.034.2083521
TCCAGTC132600.034.19008625
CCAGTCA133350.033.930326
TCGGAAG140000.033.6850053
AGAGCAC138950.033.5352828
CTCGTAT132400.033.5300944
CACATGT135600.033.4011131
GTCACAT136900.033.16557729
AGTCACA137300.033.01970328
TCACATG138500.032.89668730
Produced by FastQC (version 0.11.2)