FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6179-t3hop1-160326.3510000005775e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6179-t3hop1-160326.3510000005775e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19043380
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2178931.1441928901276979TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG251700.042.380411
GATCGGA254850.042.319831
ACACGTC254600.041.78289813
CACACGT256150.041.60922612
CACGTCT256950.041.39200614
ACGTCTG259200.040.9977615
CGTCTGA262200.040.56300416
AGCACAC263350.040.50559210
CCGATGT259350.040.2718733
GAGCACA269400.039.6624459
CGTATGC263150.039.63130644
GAACTCC266400.039.56835621
TATCTCG263850.039.5513339
GTATGCC264350.039.4258745
CAGTCAC265850.039.41324627
GTCACCG265500.039.3551429
AGAGCAC272650.039.30528
TCGGAAG275100.039.2740173
CACCGAT267300.039.1326231
TCCAGTC268000.039.10545325
Produced by FastQC (version 0.11.2)