FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sea220.3510000005806e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sea220.3510000005806e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4699493
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1104522.350296085130885TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC127900.042.97761533
ACGTCCG129400.042.809832
TCGTATG131800.042.52397545
TCCGCAC128500.042.51429435
GTCCGCA129150.042.49196234
ACACGTC133100.042.22696713
CACGTCC131200.042.2053231
GCACACG134150.042.09906411
CCGCACA129850.042.0896236
AGTCACG133500.042.0835928
CTCGTAT132800.042.01739544
ACGTCTG133750.041.97128715
CGTCTGA133950.041.9590116
CGCACAT131000.041.92623537
TCACGTC132600.041.91109530
GTCACGT133250.041.89238429
CACGTCT134600.041.68952614
CACACGT135900.041.4898412
CAGTCAC136000.041.26036527
ACTCCAG135550.041.23091523