FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sea219.35100000057ff6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sea219.35100000057ff6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5470013
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG578971.0584435539732722TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG72250.041.2004535
CCGTCCC73700.040.51197434
CACCCGT74200.040.51151731
GTCCCGA72550.040.3178936
ACCCGTC74950.040.07611532
CCCGTCC74800.039.91584433
GTCACCC76300.039.57309329
TCACCCG76200.039.53644630
ACACGTC78450.039.2919513
GCACACG79000.039.248411
CCCGATC74050.039.19697638
AGTCACC77300.039.0029428
ACGTCTG79200.038.9479215
CACACGT80100.038.679912
CTCGTAT75000.038.39907544
CACGTCT80200.038.37812414
TCGTATG75250.038.2718545
CGTCTGA81850.037.60446516
GAACTCC81650.037.53124221
GATCTCG77700.037.2684641
Produced by FastQC (version 0.11.2)