FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sea218.35100000057f8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sea218.35100000057f8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373254
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG638764.651433747871843TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT71650.043.77610812
CACGTCT71600.043.7108114
GCACACG72150.043.566311
ACACGTC72050.043.4706213
ACGTCTG72350.043.31989315
TCGGAAG72750.043.1760643
AGCACAC73600.042.89142210
CGTCTGA72900.042.86960616
AGAGCAC73750.042.8346948
GTATCTC71150.042.75398340
TCGTATG71050.042.6890445
GTCACAG71900.042.6835229
GATCGGA72050.042.6679881
CCGTATC70900.042.6508638
AGTCACA72200.042.59965528
CGTATCT71150.042.53262339
GAGCACA74200.042.5142679
CTCGTAT71350.042.50800344
TCTGAAC73450.042.48732818
CAGTCAC72550.042.4251627