FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sea217.35100000057f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sea217.35100000057f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493681
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1070517.166925200226822TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC119450.044.15403713
CACACGT119800.044.11895412
CACGTCT120000.043.91269714
GCACACG120400.043.9005611
TCGGAAG121400.043.756973
TCGTATG119100.043.714145
CGTCTGA120300.043.7096716
AGCACAC121350.043.64959310
GATCGGA118250.043.6448251
ACGTCTG120900.043.52997215
CTCGTAT119200.043.52642444
CACAGTC120000.043.4799931
AGAGCAC122500.043.366958
AGTCAAC119850.043.3466834
GAGCACA122500.043.3118489
TCTCGTA120050.043.293243
GTCACAG120850.043.28587729
GAAGAGC123200.043.265216
ACAGTCA120450.043.24283232
CTGAACT121550.043.2402119
Produced by FastQC (version 0.11.2)