FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb9.35100000057d64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb9.35100000057d64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24230634
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3954871.6321776805344839TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC454250.041.96318444
AGTCACG455800.041.92380528
GTATGCC454850.041.88370545
ACACGTC467350.041.28763213
CTCGTAT461100.041.242242
CACGATC462950.041.06323631
ACGTCTG468400.041.0600715
CACGTCT469850.040.98124314
GTCACGA465500.040.96803729
CACACGT472750.040.88775312
GCACACG473800.040.84122511
TCGTATG468300.040.80029343
CGTCTGA475450.040.45596316
GATCTCG471550.040.4525439
CGATCAG470450.040.3894733
CAGTCAC474350.040.26992827
CCAGTCA476000.040.04036326
GAACTCC480450.039.8193621
TCACGAT479850.039.7428830
ACTCCAG482000.039.6866423
Produced by FastQC (version 0.11.2)