FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb8.35100000057cf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb8.35100000057cf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20867558
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1282860.6147628773812441TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC152500.039.8352744
GTATGCC159050.038.26605245
GCACACG166250.037.29966711
ACACGTC166200.037.28309613
TCGTATG164000.037.16559243
CTCGTAT166800.036.47435442
GTCACAC168250.036.32022529
CACACGT171100.036.281312
ACGTCTG170850.036.1494115
CACGTCT171250.036.07819714
CAGTCAC171250.035.6837927
CGTCTGA173950.035.4405116
ACTCCAG175650.034.94362623
CCAGTCA175450.034.82957526
GAACTCC178000.034.65925621
TCCAGTC182000.033.6255425
TCTCGTA183400.033.2711141
AGCACAC189600.032.93153810
AGTCACA186400.032.8802528
CTCCAGT186650.032.82399424
Produced by FastQC (version 0.11.2)