FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb7.35100000057c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb7.35100000057c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18987862
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1117490.5885286084341671TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC148800.039.46499344
GTATGCC154300.038.0584745
CTCGTAT156150.037.507142
TCGTATG157000.037.46118543
AGTCACC158100.037.04400328
ACACGTC159650.036.980613
ACGTCTG159250.036.77620315
CACGTCT161050.036.56075714
GCACACG163000.036.41506611
CACACGT164150.036.2548512
CGTCTGA164750.035.57578716
CAGTCAC167100.035.38524627
CCAGTCA170250.034.7039326
CCAGATC169550.034.50333
ACTCCAG170750.034.496823
ACCAGAT169950.034.47484232
GTCACCA170450.034.4391729
CATCTCG172150.034.13873739
GAACTCC172550.034.1369421
TCCAGTC175150.033.65588825
Produced by FastQC (version 0.11.2)