FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb5.35100000057bb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb5.35100000057bb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29260674
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2333230.7973944824374175TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC294200.040.32686245
CGTATGC295650.040.09843444
TCGTATG306850.038.7007743
ACGTCTG308600.038.6777615
CACGTCT311350.038.43731314
ACACGTC311900.038.42081513
GATCTCG308100.038.24480439
CTCGTAT310500.038.00696642
CGTCTGA315400.037.85100616
GCACACG318950.037.76365311
ACTCCAG314850.037.33797523
CACACGT322550.037.33404512
CAGTCAC315100.037.30140327
GTCACAC316250.037.10198629
CCAGTCA318600.036.8138226
CACAGTG322450.036.4102833
AGTGATC321950.036.404336
TCCAGTC323250.036.31202325
GAACTCC327400.036.16099521
ACACAGT328150.035.8533132
Produced by FastQC (version 0.11.2)