FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb4.35100000057b4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb4.35100000057b4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17111096
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC722670.42233998336518014TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC99600.036.3698944
GTATGCC102150.035.55039645
CTCGTAT106150.034.2322642
TCGTATG107700.033.73920443
ACGTCTG110650.033.51013615
ACACGTC111850.033.27179713
CACGTCT112150.033.08209614
GCACACG113450.032.9626611
ACTGACC110700.032.76398532
CAGTCAC112400.032.36786727
CCAGTCA113750.032.0034126
CACACGT116950.031.89791312
CGTCTGA117150.031.68925716
CTGACCA114700.031.50368933
GTCACTG115450.031.4936429
ACTCCAG117450.031.37834223
TCCAGTC117500.031.17350625
GAACTCC122000.030.37406221
TCTCGTA121650.030.00003841
CACTGAC122500.029.86508431
Produced by FastQC (version 0.11.2)