FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb2.35100000057a4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb2.35100000057a4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782069
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC78460.10082151674573947TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16400.024.96888544
GTATGCC17850.023.06679545
CTCGTAT18950.021.37190642
TCGTATG19450.021.16913443
CGATCTC18950.021.01584238
ACGTCTG19650.020.83930415
CACGATC19150.020.20927836
CAGTCAC20100.020.0369427
GATCTCG20650.019.93947839
ACGATCT20450.019.14463437
CACGTCT21500.019.04615414
CACACGT21400.019.03050812
CGTCTGA21800.018.68084116
GCACACG22250.018.6069911
ACTCCAG22400.018.18022723
ACACGTC22200.018.14178513
CCAGTCA23550.017.38809826
GAACTCC23850.017.35794321
CATCACG23100.017.33778433
TCCAGTC24400.017.05888725
Produced by FastQC (version 0.11.2)