FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb13.35100000057ea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb13.35100000057ea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17967005
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2375451.3221179601163355TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC278550.041.4613745
CGTATGC279100.041.36308344
GCACACG286700.040.85768511
AGTCACC281850.040.83189828
ACGTCTG284750.040.79554715
ACACGTC286050.040.79150413
GTCACCT282400.040.7523729
CACACGT288050.040.64129612
CTCGTAT284700.040.51064342
CACGTCT288700.040.3464914
TCGTATG286900.040.30150243
CAGTCAC289250.039.9737427
CACCTTG289450.039.8541731
CGTCTGA291700.039.846716
CCTTGTA289350.039.79796233
GAACTCC297150.039.16843821
ACCTTGT294750.039.16807632
CCAGTCA295200.039.1527926
AGCACAC302650.038.7713510
TCTCGTA298850.038.71299741
Produced by FastQC (version 0.11.2)