FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb11.35100000057e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb11.35100000057e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19159744
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1294260.6755100694456043TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA161950.039.94260832
GTCACGG163500.039.67321429
TCACGGC162850.039.5276130
CGGCTAC162100.039.50312433
CACGGCT163850.039.42450731
AGTCACG166750.038.85949328
CGTATGC165350.038.84853744
GGCTACA168200.037.9369234
GTATGCC169350.037.91795745
ACACGTC174600.037.56437313
CTCGTAT171150.037.50631742
GCACACG177700.037.13832511
TCGTATG173700.037.0846643
ACGTCTG176950.036.97600615
GCTACAT174500.036.6704335
CACACGT180500.036.54851512
CACGTCT179850.036.46735414
CGTCTGA183350.035.7344116
CAGTCAC181500.035.68909527
CTACATC180700.035.2884536
Produced by FastQC (version 0.11.2)