FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l05n01_sb10.35100000057dc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l05n01_sb10.35100000057dc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21378722
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC6521133.050289909752323TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC725200.043.15139831
ACTAGCT730600.042.91868632
GTATGCC732750.042.77650545
CGTATGC734150.042.68563544
ACACGTC743600.042.6820613
ACGTCTG744250.042.54115
TAGCTTA736350.042.46151434
CACGTCT745950.042.4500814
TCACTAG738700.042.42526630
GCACACG749600.042.4198511
CACACGT749400.042.37913512
CTCGTAT737900.042.34654642
CGTCTGA749150.042.24172216
CTAGCTT741450.042.22397233
TCGTATG745850.042.08220343
GCTTATC740950.042.07967436
CAGTCAC746750.042.05198727
CCAGTCA747350.041.9880226
GAACTCC752950.041.9177821
CTTATCT746450.041.8178537