FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea216.351000000582c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea216.351000000582c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13487804
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC136071110.088454725469024TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1491850.044.20261813
CACGTCT1490250.044.1804614
GTATGCC1482450.044.16740845
ACGTCTG1491350.044.15945415
CACACGT1496100.044.14688512
CGTCTGA1491200.044.14428316
GCACACG1498600.044.1307811
AGCACAC1511500.043.83006310
AGTCACC1489400.043.76570528
CGTATGC1496900.043.74523544
GTCACCT1487050.043.7304729
GAACTCC1501900.043.70710421
CTCGTAT1495800.043.70496442
CCTTGTA1486400.043.67963833
ACTCCAG1498800.043.6759123
TCTGAAC1509400.043.6196118
TCGTATG1501650.043.6191443
CCAGTCA1498300.043.59636726
CAGTCAC1501500.043.59186627
GTAATCT1493800.043.58590337
Produced by FastQC (version 0.11.2)