FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea215.35100000058279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea215.35100000058279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10031688
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2957172.9478289197191936TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC330150.043.3378233
ACGTCTG337450.043.24451415
ACGGCTA334650.043.2057532
GTCACGG337600.043.06814629
ACACGTC339700.042.98543513
CGTATGC336700.042.91831644
CTCGTAT336550.042.89518742
GCACACG341500.042.89127311
GGCTACA332450.042.82818234
GTATGCC337700.042.82560745
TCACGGC339100.042.79137430
AGTCACG340750.042.7822628
CACGGCT338600.042.78146731
CACACGT343100.042.6385812
CGTCTGA342700.042.58859316
CACGTCT342750.042.5698914
TCGTATG340000.042.56432743
GCTACAT335900.042.4146735
GATCGGA349350.042.1131441
GAACTCC345600.042.06196221