FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea214.35100000058236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea214.35100000058236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10195286
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC209550.20553616642044176TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC31300.032.92352331
CGTATGC33450.031.54959944
ACTAGCT34100.030.55004132
ACGTCTG35450.030.14665415
CTCGTAT35150.029.82977742
GTATGCC36300.029.0729945
GCACACG37250.028.81225811
TAGCTTA37100.028.32199734
CACGTCT37850.028.29510714
CTAGCTT37450.027.93714533
TCGTATG38200.027.85929743
ACACGTC38550.027.8396813
CACACGT39100.027.44861212
CGTCTGA40600.026.59971416
CAGTCAC39900.026.44688227
TCACTAG40700.025.65126430
GCTTATC41200.025.3402136
GAACTCC42650.024.95191221
TCCAGTC43600.024.71797425
CCAGTCA43150.024.55922326
Produced by FastQC (version 0.11.2)