FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea213.351000000581e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea213.351000000581e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864509
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3286254.787305253733369TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC357700.043.8576545
ACACGTC361250.043.7040913
ACGTCTG360250.043.6870515
CGTATGC359500.043.624944
AGTCACG358650.043.61314428
CGTCTGA361700.043.51191316
GCACACG364000.043.48643511
CTCGTAT360550.043.42742
CACGTCT363150.043.39488214
CACACGT364300.043.38886312
CACGATC357700.043.31382431
GTCACGA359550.043.27869429
TCGTATG362500.043.2734543
CGATCAG358450.043.12276533
GATCTCG362250.043.0992939
CAGTCAC363900.043.08872627
GAACTCC365250.043.04588721
ACTCCAG365500.043.00412823
CCAGTCA365550.042.90039426
CAGATCT361050.042.86862637