FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea212.35100000058187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea212.35100000058187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10153262
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC131387112.940383100524738TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1421950.044.46447411
ACACGTC1421550.044.4322313
CACGTCT1423700.044.33194714
CACACGT1423650.044.33192412
ACGTCTG1423200.044.31568515
CGTCTGA1424250.044.28279516
AGCACAC1430300.044.26309610
GTATGCC1407100.044.22289745
GATCGGA1429950.044.21811
TCGGAAG1433400.044.095983
GAACTCC1430400.044.05779621
ACTCCAG1425150.044.049623
CGGAAGA1446150.044.0471274
AGAGCAC1443350.044.023248
TCCAGTC1426550.043.95747425
GTCACAC1421000.043.9252729
CAGTCAC1426450.043.9219927
CGTATGC1417750.043.90138244
CCAGTCA1427150.043.89414226
CTCGTAT1416750.043.88371342
Produced by FastQC (version 0.11.2)