FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea211.35100000058137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea211.35100000058137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10397250
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC9409889.050354661088269TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1045600.044.2812711
ACACGTC1045450.044.25491313
CACGTCT1045500.044.17941314
CACACGT1047700.044.14526712
ACGTCTG1046300.044.0912415
AGCACAC1053450.043.9876110
CGTCTGA1049000.043.9713216
GATCGGA1060200.043.927141
GTATGCC1046850.043.88185545
TCGGAAG1062550.043.82563
CGGAAGA1073200.043.6573754
AGAGCAC1068650.043.6272358
ACTCCAG1047800.043.59651623
CGTATGC1053750.043.58685344
GAACTCC1052150.043.5787921
CCAGTCA1046300.043.50890426
TCCAGTC1048050.043.48736225
GAAGAGC1076450.043.4398656
CAGTCAC1051100.043.4322327
CTGAACT1061500.043.42384719
Produced by FastQC (version 0.11.2)