FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea210.351000000580d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea210.351000000580d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8962956
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4721685.2679941751359705TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG558500.043.73967711
ACACGTC557100.043.6787513
CACACGT558650.043.67081512
ACGTCTG558250.043.57143415
CACGTCT558050.043.5358514
GTATGCC550900.043.45514745
CGTCTGA561400.043.33096316
AGCACAC564650.043.2871910
CGTATGC555900.043.06332844
GATCGGA573150.043.0514871
CAGTCAC562350.043.02290727
CTCGTAT554050.042.97371342
GAACTCC563850.042.9591321
ACTGACC556600.042.9021532
CCAGTCA562750.042.86438426
TCCAGTC563700.042.8109125
CTGACCA555450.042.8001133
TCGTATG560400.042.7711243
ACTCCAG564850.042.75959823
CGGAAGA577750.042.7557754