FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea209.35100000058052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea209.35100000058052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6005185
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3618026.024826878772261TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG396200.043.98308611
GTATGCC392550.043.96656445
ACACGTC395300.043.94625513
ACGTCTG395200.043.93956415
CACGTCT395650.043.90169514
ACTTAGG390000.043.88615832
CACACGT396600.043.8702812
CACTTAG390550.043.86467731
CGTCTGA396750.043.76790216
CGTATGC394800.043.70277444
GATCGGA398550.043.618991
AGCACAC400900.043.51795610
AGGCATC390900.043.51603336
TCGGAAG399250.043.5136453
CTCGTAT396150.043.46151742
GGCATCT391500.043.4550937
CAGTCAC397650.043.4149527
TCGTATG398650.043.3395543
TTAGGCA393400.043.30097234
CCAGTCA398250.043.29304526