FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea208.35100000057fe9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea208.35100000057fe9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9027170
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC161756717.91887158433928TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1742850.044.58035711
CACACGT1741600.044.53693412
ACACGTC1741400.044.5314613
CACGTCT1740900.044.4692914
GATCGGA1744850.044.4671021
ACGTCTG1740900.044.4385815
TCGGAAG1748550.044.3683053
CGTCTGA1744500.044.35590416
AGCACAC1753000.044.35560610
GTATGCC1729600.044.33439645
CGGAAGA1763950.044.3004384
AGAGCAC1763000.044.2724768
GAAGAGC1769800.044.175136
TCTGAAC1751750.044.11324718
GAGCACA1766550.044.1096349
GAACTCC1749750.044.1058121
ATCGGAA1760850.044.097212
ACTCCAG1747100.044.07612223
CTGAACT1754400.044.069719
CGTATGC1740950.044.04931344
Produced by FastQC (version 0.11.2)