FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea207.351000000579d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea207.351000000579d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926823
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1676415.727746433590278TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT193000.044.06677212
ACACGTC192100.044.0506913
CACGTCT192450.043.9004314
GCACACG193950.043.79292311
ACGTCTG193500.043.5691915
CGTCTGA193850.043.42088316
TCGGAAG196100.043.407543
CTCGTAT193100.043.37979542
AGCACAC195800.043.37914710
GATCGGA196400.043.3708151
GAACTCC193600.043.3374921
AGTCACC192500.043.3053628
TCGTATG194900.043.19850543
GTATGCC194650.043.19840245
AGAGCAC197600.043.1775678
CGTATGC194750.043.1646744
TCTCGTA194700.043.0464241
GAGCACA198100.043.0458689
TCCAGTC195250.042.8790725
CAGTCAC195400.042.8576727
Produced by FastQC (version 0.11.2)