FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_sea206.35100000057968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_sea206.35100000057968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790836
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC778912.7909558282894444TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC84550.043.0849213
CACACGT84900.042.93380412
CTCGTAT83800.042.905942
CACGTCT85250.042.73037314
TCGTATG84650.042.5798643
AGTCACG84550.042.5785328
CGTATGC85050.042.46124644
ACGTCTG85850.042.37779615
GCACACG86300.042.36842711
GTATGCC85400.042.31357645
TCTCGTA85200.042.2272841
CAGTCAC85950.042.09365527
TATCTCG85500.042.07911739
GTCACGC85100.041.9331929
GAACTCC86950.041.89343321
CGTCTGA87100.041.8471216
AGCACAC87450.041.81126810
ATCTCGT86700.041.54860740
CCAGTCA87050.041.53589626
GATCGGA87750.041.44721