FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp77.35100000057f7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp77.35100000057f7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25237140
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG674790.26737974271252607TruSeq Adapter, Index 27 (97% over 44bp)
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTAC344830.13663592625788817No Hit
GATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG284240.11262765907705866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG89550.040.80445511
CACACGT93550.038.96291712
GAGCACA98950.037.246489
GAACTCC97550.037.0642421
ACACGTC98500.036.95905313
TATCTCG89250.036.95837841
ACTCCAG99800.036.04826723
GATCGGA107850.033.7658121
CAGTCAC102900.033.76015527
ACGTCTG110450.033.0612515
AGCACAC112500.032.96035410
CGTCTGA112450.032.37319616
CACGTCT113350.032.07715214
GTCACAT105400.031.61523629
CTGAACT115500.031.57675719
AGAGCAC118400.031.4699298
AGTCACA107700.031.37879228
ACATTCC107350.030.93615232
GTCTGAA122000.029.9128317
CGGCCGG29800.029.74828730
Produced by FastQC (version 0.11.2)