FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp76.35100000057f05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp76.35100000057f05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7378964
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG854021.1573711431577658TruSeq Adapter, Index 25 (97% over 44bp)
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG101330.13732280032806773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG100250.043.04766511
CACACGT101450.042.56036412
ACACGTC101650.042.38722213
GAGCACA103000.042.3789149
GAACTCC101350.042.20131721
GTCACAC99400.041.9667929
TATCTCG97150.041.9205541
ACTCCAG102900.041.43444423
AGCACAC106350.040.95936210
ACGTCTG104900.040.94473615
GATCGGA106100.040.937511
CACACTG101350.040.8707331
CAGTCAC103850.040.73126227
AGAGCAC107600.040.713558
ACACTGA101950.040.60812832
CACGTCT106050.040.52250314
CACTGAT101550.040.4573433
CGTCTGA106500.040.35073516
CTGAACT106750.040.2351619
AGTCACA105050.039.9452528