FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp75.35100000057e9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp75.35100000057e9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845953
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG452860.5119403189232409TruSeq Adapter, Index 7 (97% over 36bp)
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG152880.1728247934394406No Hit
GGAGGAAGTGATGTTGCTAGAGGAAGATGGGGTTGGTACTGGTGCTACAGA91470.10340321726782858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG59600.041.79164511
CACACGT60100.041.29420512
ACACGTC60950.040.60688813
GAACTCC61500.040.4252921
ACTCCAG62150.040.00250223
AGTCACG59100.039.97445328
TATCTCG57600.039.68823241
CGAGTGG57350.039.31086333
GAGCACA64050.039.2745069
ACGAGTG58300.038.8248832
CAGTCAC63600.038.70157627
GTCACGA60900.038.20181329
CACGAGT59450.038.03600731
CACGTCT65550.037.85982514
CTGAACT66400.037.5098719
ACGTCTG66650.037.20038215
AGCACAC68300.036.92946210
CGTCTGA68100.036.57349416
AGAGCAC69350.036.3378838
GATCGGA69150.036.095261
Produced by FastQC (version 0.11.2)