FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp74.35100000057e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp74.35100000057e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7206579
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG483690.6711783774242952TruSeq Adapter, Index 22 (97% over 40bp)
GGAGACAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCTTC104830.14546430421424644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG55500.043.01321433
GCACACG59450.042.69074611
CGTACGT56050.042.51085334
CACACGT59850.042.4430212
ACACGTC61050.041.49819613
GAACTCC61050.041.3504921
GAGCACA61700.041.061029
GATCGGA61850.040.936421
ACTCCAG61900.040.6372823
CACGTCT64300.039.36570414
CAGTCAC63350.039.28149427
AGCACAC65300.039.14187610
ACGTCTG65600.038.7225215
CGTCTGA65550.038.68340716
GTACGTA62300.038.4989335
AGAGCAC67350.037.9504788
TACGTAA63650.037.50615336
GTCTGAA67800.037.49921817
CTGAACT68000.037.25657719
TCTCGTA66200.036.29884343
Produced by FastQC (version 0.11.2)