FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp73.35100000057db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp73.35100000057db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7934722
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG164740.20761912011536132TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24950.039.95079411
CACACGT26450.037.7690312
ACACGTC28100.035.5508313
GAACTCC28750.035.0594521
ACTCCAG29150.034.19243623
GAGCACA29900.033.8636369
AGTCACG27100.033.29347228
CAGTCAC30200.032.1108427
CGTTTCG27150.031.98845733
GATCGGA31300.031.784691
ACGTCTG31950.031.54801415
AGCACAC32900.031.32289110
CGTCTGA32600.030.98800716
CACGTCT33000.030.2720714
AGAGCAC34100.030.0226698
GTCACGT29700.029.84858929
CTGAACT34850.029.05190519
CTCCAGT34850.028.72910524
GTCTGAA35300.028.36287117
AACTCCA37650.027.19013222
Produced by FastQC (version 0.11.2)