FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp72.35100000057d3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp72.35100000057d3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567986
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG465490.48650781888685873TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57150.041.41806811
CACACGT57800.041.02993812
ACGTGGC55250.040.96820432
CACGTGG55550.040.7469631
TCACGTG55850.040.52808430
ACACGTC58900.040.18663813
TATCTCG57200.039.6504441
CGTGGCC57100.039.4832533
GAACTCC60850.039.0083221
GAGCACA61200.038.8977629
AGTCACG59450.038.7929928
ACTCCAG61450.038.55421423
GTCACGT60500.037.45030229
CAGTCAC62350.037.34855327
CGTCTGA64200.036.97284316
CACGTCT64300.036.91649614
ACGTCTG64550.036.84207515
AGCACAC65500.036.5846310
GATCGGA66100.035.684681
AGAGCAC68700.034.9460458
Produced by FastQC (version 0.11.2)