FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp71.35100000057cc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp71.35100000057cc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8834083
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG106530.12058976579685746TruSeq Adapter, Index 19 (97% over 40bp)
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTAC89340.10113103986005112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTG15850.034.77958330
GCACACG17350.033.71777711
CACGTGA17050.032.7276531
ACACGTC18250.030.822113
GAACTCC18650.030.40094821
CACACGT19400.029.92285712
ACTCCAG19950.028.75826623
AGTCACG19650.028.2827728
ACTCGGG11300.027.67590118
AGCCCGT11250.026.59985514
AAGCCCG11400.026.44752513
GATCTCG20500.026.122441
GAGCACA22700.025.6719599
CAGTCAC21800.025.59546727
GCCGGCT6250.025.5679661
CGATCTC20700.025.54377440
CTCGGGC12600.024.82045219
AGCACAC25250.024.0595510
GTCACGT23350.023.70477129
CTCGTAT22400.023.60598644
Produced by FastQC (version 0.11.2)