FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp70.35100000057c59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp70.35100000057c59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10139322
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGACAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCTTC163310.1610659963259871No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG129390.12761208293808995TruSeq Adapter, Index 18 (97% over 40bp)
GATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG106630.10516482265776744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21350.037.096511
CGTCCGC18400.037.05137633
ACGTCCG19050.036.49599532
GTCCGCA20450.033.66741634
ACACGTC23850.033.11314813
CACACGT24250.032.845312
CGCACAT21500.032.23313537
GAGCACA25100.032.1816949
CCGCACA21550.031.94952836
GAACTCC24050.031.71402421
ACTCCAG24450.031.28720723
AGTCACG23650.030.7293328
ACTCGGG10100.030.07316218
CATCTCG24050.030.03172141
GATCGGA26850.029.923231
GTCACGT24050.029.75046529
TCACGTC24200.029.47308330
TCCGCAC23900.028.80733535
CGTCTGA27500.028.79895216
CTCGGGC10800.028.33230219
Produced by FastQC (version 0.11.2)