FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp69.35100000057bed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp69.35100000057bed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520460
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG45700.8780694001460247TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG5050.042.7723735
ACCCGTC5300.042.4529232
ACACGTC6300.042.13889713
GCACACG6300.041.785811
CACGTCT6300.041.78178814
CACCCGT5500.041.72736431
CCCGTCC5450.041.2844933
TCACCCG5650.041.01778830
CGTCTGA6450.040.81011616
CCGTCCC5500.040.50008834
CACACGT6500.040.49619712
CAGTCAC5950.040.46227327
TCTGAAC6500.040.15007818
GAACTCC6450.040.11250721
ACGTCTG6600.039.88261415
TCCCGAT5200.039.81160437
GTCCCGA5200.039.81160436
ACTCCAG6450.039.76370223
AGCACAC6750.039.66674810
GAGCACA6750.039.6667489
Produced by FastQC (version 0.11.2)