FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp68.35100000057b5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp68.35100000057b5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4978437
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG806351.6196850537628575TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG97500.043.29075211
CACACGT98550.042.82908212
GAGCACA99550.042.7156949
ACACGTC99250.042.61768713
GATCGGA101050.042.4549181
TCACATG98350.042.0040730
CACGTCT100600.041.93395614
GTCACAT98900.041.92973729
ACGTCTG101350.041.7564215
AGTCACA99850.041.73361628
ACTCCAG100600.041.62043423
AGCACAC102100.041.47255710
CGTCTGA102450.041.2861216
CACATGT100200.041.16118231
TCTGAAC102900.040.97438418
ATCGGAA104600.040.9342772
CTGAACT103200.040.8770719
CAGTCAC102150.040.8135127
GTCTGAA104350.040.57750717
TCGTATG99450.040.5476445
Produced by FastQC (version 0.11.2)