FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp67.35100000057ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp67.35100000057ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4938047
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG705171.4280342005655273TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG84200.043.1029811
CACACGT84250.042.99684512
ACACGTC84700.042.5820313
GATCGGA85850.042.3389851
GAGCACA86400.042.2398349
TATCTCG81550.042.10367641
TCCGTAT81650.041.9418837
CCGTATC81450.041.87912438
CACGTCT85900.041.7776314
CGTCTGA85700.041.7688416
GAACTCC85900.041.67201221
ACTCCAG85850.041.6438723
GTTCCGT82050.041.6268835
AGTCACA84750.041.54891628
AGTTCCG82850.041.38829434
GTCACAG84100.041.3349629
AGCACAC88200.041.3012610
ACGTCTG86850.041.24167615
CAGTCAC86150.041.16017527
GTCTGAA88350.040.61828617
Produced by FastQC (version 0.11.2)