FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l04n01_mrp66.35100000057a5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l04n01_mrp66.35100000057a5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5591129
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG591411.057764898645694TruSeq Adapter, Index 13 (97% over 40bp)
GGAGACAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCTTC69190.12374960406028906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58590.10479100017187941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG72500.042.61078611
GAGCACA72600.042.5830889
GATCGGA73250.042.160371
GAACTCC72650.042.08658221
CACACGT73800.041.76835312
ACTCCAG73150.041.64512323
GTCACAG73000.041.24015429
ACACGTC75400.040.88092413
AGTCACA74350.040.7031828
CACGTCT76000.040.439414
CGTCTGA76200.040.42111216
ACGTCTG76700.040.18694715
CAGTCAC75850.040.10444627
TCGTATG74200.040.03166645
AGCACAC79100.039.25451310
CTCGTAT75700.039.1184844
GTCTGAA78900.039.00936517
CTGAACT79100.038.8822919
TCTGAAC79650.038.52906418
AACTCCA79750.038.367922
Produced by FastQC (version 0.11.2)