FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l01n01_sv122.35100000057869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l01n01_sv122.35100000057869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33185259
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC5920741.784147593966345TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA677650.042.51881416
CTCGTAT668150.042.45846642
TCTCGTA670050.042.42745241
CGTATGC669650.042.34668444
TCGTATG670300.042.34235443
AATCTCG671950.042.3311239
ACACGTC684900.042.22306413
ACGTCTG684200.042.21035815
ATCTCGT680350.041.7687940
CACGTCT699400.041.36699314
GTATGCC690900.041.0442945
CACACGT711450.040.7770412
GCACACG712950.040.7764611
ATCGGAA728550.040.5460242
CGGAAGA726700.040.5054664
TCGGAAG729550.040.412913
GATCGGA731350.040.367681
GTAATCT711500.039.94960837
TGTAATC733900.038.77012636
AGTCACC732600.038.7062228