FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l01n01_sv121.35100000057826.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l01n01_sv121.35100000057826.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27203451
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2124600.7810038513128352TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT250950.041.1376142
TCTCGTA253550.040.7403741
TCGTATG254400.040.6591143
CGTATGC256750.040.23467644
ATCTCGT259900.039.80565640
CATCTCG268800.038.5546539
GTATGCC275650.037.50869845
CGTCTGA286450.036.85361516
ACACGTC289950.036.58729613
GATCATC285000.036.3392436
ACGTCTG293200.036.08191715
CACGTCT301250.035.22976714
ATCGGAA306750.035.0116122
GATCGGA309650.034.6809731
AGATCAT305250.033.9432735
CACACGT315700.033.63163412
TCGGAAG320950.033.441173
CGGAAGA326000.032.8950464
ACCAGAT317350.032.61308332
GCACACG328250.032.38691711
Produced by FastQC (version 0.11.2)