FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l01n01_sv120.351000000577c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l01n01_sv120.351000000577c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29617491
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1721970.5814030634802928TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT204800.040.89261242
TATCTCG206000.040.7947539
TCTCGTA205750.040.71288341
CGCCAAT204650.040.65610533
TCGTATG206750.040.53951343
CGTATGC210450.039.81641444
ACGCCAA209250.039.7946132
ATCTCGT213550.039.32085440
GTCACGC214550.038.9585129
CGTCTGA222850.038.4866516
ACACGTC225600.038.147113
TCACGCC219900.037.96975330
AGTCACG223350.037.42354628
ACGTCTG229950.037.41567615
CACGCCA226700.036.8705331
CACGTCT240450.035.9034514
GTATGCC233950.035.84575745
GCCAATA234200.035.73788534
ATCGGAA248750.035.142172
CAATATC240550.034.7855436
Produced by FastQC (version 0.11.2)