FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l01n01_sv119.35100000057777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l01n01_sv119.35100000057777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33882839
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC22718316.704960584914386TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2590500.044.1194531
ATCGGAA2592100.044.104752
TCGGAAG2594350.044.0081253
ACACGTC2540450.043.98126613
CGGAAGA2603600.043.7938354
CGTCTGA2543400.043.7613616
ACGTCTG2552550.043.75074415
GCACACG2572800.043.63564311
CACGTCT2563050.043.61452514
CACACGT2571600.043.56413712
CTCGTAT2543100.042.87762542
AATCTCG2556100.042.87301339
TCTCGTA2548300.042.8627841
TCGTATG2547200.042.81561343
CGTATGC2544850.042.7814544
ATCTCGT2557100.042.69874640
GTATGCC2548500.042.68498645
GAGCACA2665200.042.3533029
GAACTCC2595500.042.31637221
GACCAAT2585750.042.24379335
Produced by FastQC (version 0.11.2)